How kinases control membrane trafficking
We investigate how membranes of the secretory pathway are regulated by signal transduction pathways. Here, we are particularly interested in the role of the protein kinase D family. Specifically, we address how external cues control PKD activity and how the kinase engages its interactors and substrates to coordinate the secretory pathway.
Ultimately, we aim at determining the relevance of PKD in cell growth, polarity, migration and invasion, and stem cell maintenance to understand the impact of dys-regulation of PKD signaling and secretion in human diseases such as cancer and neurodegeneration.
microRNAs in cell invasion
Another research area in the lab is the investigation of the role of microRNAs in invasive cell migration. In an image-based screen we have identified miRNAs crucial for invasive migration of human macrophages. We are now particularly interested how these miRNAs regulate the pro-invasive crosstalk of macrophages and breast cancer cells.
We employ an interdisciplinary approach combining 2D and 3D cell culture including primary cells from mice and humans, biochemistry, advanced microscopic techniques e.g. TIRF, FRET, and FRAP, image-based high-throughput screening, and in vivo mouse models.
Our latest research paper "A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions" has just been published in eLife! In this paper, we identify a microtubule-regulated Rho signaling network connecting extracellular cues with PKD-controlled secretory transport. The article is available online: https://elifesciences.org/articles/35907
A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions. Eisler SA, Curado F, Link G, Schulz S, Noack M, Steinke M, Olayioye MA, Hausser A. Elife. 2018 Jul 20;7. pii: e35907. doi: 10.7554/eLife.35907.
- Protein kinase D exerts neuroprotective functions during oxidative stress via nuclear factor kappa B-independent signaling pathways. Liliom H, Tárnok K, Ábrahám Z, Rácz B, Hausser A, Schlett K. J Neurochem. 2017 Jul 19. doi: 10.1111/jnc.14131. [Epub ahead of print]. PMID: 28722750
- Coordination of AMPA receptor trafficking by Rab GTPases. Hausser A, Schlett K.Small GTPases. 2017 Jun 19:1-14. doi: 10.1080/21541248.2017.1337546. [Epub ahead of print]. PMID: 28628388
- ATF6β-based fine-tuning of the unfolded protein response enhances therapeutic antibody productivity of Chinese hamster ovary cells. Pieper LA, Strotbek M, Wenger T, Olayioye MA, Hausser A. Biotechnol Bioeng. 2017 Jun;114(6):1310-1318. doi: 10.1002/bit.26263. PMID: 28165157
- Secretory pathway optimization of CHO producer cells by co-engineering of the mitosRNA-1978 target genes CerS2 and Tbc1D20.Pieper LA, Strotbek M, Wenger T, Gamer M, Olayioye MA, Hausser A. Metab Eng. 2017 Mar;40:69-79. doi: 10.1016/j.ymben.2017.01.003. Epub 2017 Jan 11. PMID: 28088541
- Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover. Szíber Z, Liliom H, Morales CO, Ignácz A, Rátkai AE, Ellwanger K, Link G, Szűcs A, Hausser A, Schlett K. Mol Biol Cell. 2017 Jan 15;28(2):285-295. doi: 10.1091/mbc.E16-07-0526. Epub 2016 Nov 16. PMID: 27852895
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Our lab on ResearchGate
Angelika Hausser, Lab head
Sebastian Lieb, PhD student
Carlos Omar Oueslati Morales, PhD student
Gisela Link, Technical assistant
Stefanie Föller (Master Student)
Kai Hirzel (Master Student)
Viktória Szentgyörgyi (ERASMUS+ Student)
Attila Ignácz (PhD Student, DAAD program PPP Hungary)
Elena Gutiérrez Galindo (Research assistant)
Applications of students and scientists interested in signal transduction and membrane trafficking, and joining our team are always welcome! Please send you CV and a short summary of your research interests to Angelika Haußer.