BIOspektrum Spezial: Zellbiologie
FRET-Biosensoren: molekulare Werkzeuge in der Signalwegforschung
Stephan A. Eisler
Genetically encoded, FRET based biosensors have revolutionized cell signalling research in the past decade. They allow tracking dynamic activities of signalling molecules such as protein kinases, Rho GTPases or second messengers in real time on a subcellular level. This article gives an overview about design, components and employment of different types of FRET biosensors. Exemplarily, a kinase activity reporter to visualize protein kinase D activity at the Golgi complex is presented.
DOI: 10.1007/s12268-013-0328-7
© Springer-Verlag 2013
Korrespondenzadresse:
Dr. Stephan A. Eisler
Institut für Zellbiologie und Immunologie
Labor für Angewandte Mikroskopie und Bildanalyse, Zentrum Systembiologie
Universität Stuttgart
Allmandring 5b
D-70569 Stuttgart
Tel.: 0711-685-68209
Fax: 0711-685-67484
stephan.eisler@izi.uni-stuttgart.de